Biotechnology Preparation

About Course

This Refresher Course helps aspiring candidates to recapitulate and improve their fundamental knowledge on various subjects pertaining to Biotechnology.

It covers the relevant topics under the academic curriculum for Graduate and Diploma level Engineering, where video lectures from world class institutions e.g. IITs, IISc etc. have been facilitated for dissemination of knowledge.

Course Content

General Biotechnology

Introduction

06:01
Good Lab Practices (Part 1)

27:06
Good Lab Practices (Part 2)

25:47
Operation of Laboratory Instruments (Part 1)

52:54
Operation of Laboratory Instruments (Part 2)

32:14
Operation of Laboratory Instruments (Part 3)

25:44
Solution and Buffer Preparation

51:26
Basics of Electrophoresis (Part 1)

01:06:09
Basics of Electrophoresis (Part 2)

42:57
Horizontal Gel Electrophoresis

29:33
Different Variants of Gel Electrophoresis

45:05
Scientific Questions (Part 1)

26:48
Scientific Questions (Part 2)

27:28
Scientific Questions (Part 3)

24:18
Scientific Questions (Part 4)

28:55
Basics of Chromatography (Part 1)

36:55
Basics of Chromatography (Part 2)

33:29
Ion-Exchange Chromatography (Part 1)

49:02
Ion-Exchange Chromatography (Part 2)

34:03
Hydrophobic Interaction Chromatography

18:44
Gel Filtration Chromatography (Part 1)

42:15
Gel Filtration Chromatography (Part 2)

35:46
Gel Filtration Chromatography (Part 3)

46:10
Affinity Chromatography (Part 1)

40:53
Affinity Chromatography (Part 2)

01:09:52
Affinity Chromatography (Part 3)

29:04
Affinity Chromatography (Part 4)

47:37
Antibody Generation

55:38
Antibody-Antigen Interaction (Part 1)

50:07
Immunoassay

46:15
Antibody-Antigen Interaction (Part 2)

45:29
Antibody-Antigen Interaction (Part 3)

41:06
Cell Culture Medium

39:52
Cell Fractionation

42:13
Microscopy (Part 1)

54:37
Microscopy (Part 2)

45:36
Cell Biology Experiments

57:42
Flow Cytometry

59:06
Polymerase Chain Reaction (Part 1)

40:33
Polymerase Chain Reaction (Part 2)

29:56
Polymerase Chain Reaction (Part 3)

42:38
Polymerase Chain Reaction (Part 4)

47:35
Sequencing Techniques

51:24
Blotting Techniques (Part 1)

40:03
Blotting Techniques (Part 2)

41:49
Designing Experiments

52:05

Recombinant DNA Technology

Theory and Application

05:48
Cellular Structure (Part I)

49:52
Cellular Structure (Part II)

01:04:58
Cellular Structure (Part III)

30:16
Metabolic Reactions in Biological System

59:43
Growth Media For Different Expression System

50:05
Microbial Growth Kinetics

50:31
Isolation of a Gene Fragment (Part I)

01:00:49
Isolation of a Gene Fragment (Part II)

41:10
Isolation of a Gene Fragment (Part III)

59:31
Polymerase Chain Reaction

45:13
Molecular Tools for Cloning

55:38
Cloning Vectors- I

32:21
Cloning Vectors- II

47:57
DNA Delivery In Host- I

01:15:40
DNA Delivery In Host- II

54:20
Screening of Recombinant Clones

01:05:56
Protein Production in Host- I

01:11:00
Protein Production in Host- II

01:00:22
Protein Production in Host- III

36:55
Product Recovery from Host Cells

50:32
Basics of Chromatography (Part 1)

31:18
Basics of Chromatography (Part 2)

41:17
Ion-exchange Chromatography

37:15
Hydrophobic Interaction chromatography

38:08
Gel Filtration chromatography (Part 1)

38:37
Gel Filtration chromatography (Part 2)

39:58
Affinity Chromatography (Part 1)

42:16
Affinity Chromatography (Part 2)

44:41
Affinity Chromatography (Part 3)

33:52
Affinity Chromatography (Part 4)

40:01
Electrophoresis (Part 1)

01:10:43
Electrophoresis (Part 2)

36:20
Electrophoresis (Part 3)

01:16:13
Protein Sequencing

27:08
Spectroscopy (Part I)

57:37
Spectroscopy (Part II)

41:20
Biotechnology Applications (Part 1)

43:07
Biotechnology Applications (Part 2)

48:22
Biotechnology Applications (Part 3)

53:20
Summary and Conclusions (Part 1)

01:03:52
Summary and Conclusions (Part 2)

59:45

Plant Biotechnology

Animal Biotechnology

Bioprocess Engineering and Process Biotechnology

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Biotechnology Preparation

About Course

What Will You Learn?

Course Content

General Biotechnology

Introduction

Good Lab Practices (Part 1)

Good Lab Practices (Part 2)

Operation of Laboratory Instruments (Part 1)

Operation of Laboratory Instruments (Part 2)

Operation of Laboratory Instruments (Part 3)

Solution and Buffer Preparation

Basics of Electrophoresis (Part 1)

Basics of Electrophoresis (Part 2)

Horizontal Gel Electrophoresis

Different Variants of Gel Electrophoresis

Scientific Questions (Part 1)

Scientific Questions (Part 2)

Scientific Questions (Part 3)

Scientific Questions (Part 4)

Basics of Chromatography (Part 1)

Basics of Chromatography (Part 2)

Ion-Exchange Chromatography (Part 1)

Ion-Exchange Chromatography (Part 2)

Hydrophobic Interaction Chromatography

Gel Filtration Chromatography (Part 1)

Gel Filtration Chromatography (Part 2)

Gel Filtration Chromatography (Part 3)

Affinity Chromatography (Part 1)

Affinity Chromatography (Part 2)

Affinity Chromatography (Part 3)

Affinity Chromatography (Part 4)

Antibody Generation

Antibody-Antigen Interaction (Part 1)

Immunoassay

Antibody-Antigen Interaction (Part 2)

Antibody-Antigen Interaction (Part 3)

Cell Culture Medium

Cell Fractionation

Microscopy (Part 1)

Microscopy (Part 2)

Cell Biology Experiments

Flow Cytometry

Polymerase Chain Reaction (Part 1)

Polymerase Chain Reaction (Part 2)

Polymerase Chain Reaction (Part 3)

Polymerase Chain Reaction (Part 4)

Sequencing Techniques

Blotting Techniques (Part 1)

Blotting Techniques (Part 2)

Designing Experiments

Recombinant DNA Technology

Theory and Application

Cellular Structure (Part I)

Cellular Structure (Part II)

Cellular Structure (Part III)

Metabolic Reactions in Biological System

Growth Media For Different Expression System

Microbial Growth Kinetics

Isolation of a Gene Fragment (Part I)

Isolation of a Gene Fragment (Part II)

Isolation of a Gene Fragment (Part III)

Polymerase Chain Reaction

Molecular Tools for Cloning

Cloning Vectors- I

Cloning Vectors- II

DNA Delivery In Host- I

DNA Delivery In Host- II

Screening of Recombinant Clones

Protein Production in Host- I

Protein Production in Host- II

Protein Production in Host- III

Product Recovery from Host Cells

Basics of Chromatography (Part 1)

Basics of Chromatography (Part 2)

Ion-exchange Chromatography

Hydrophobic Interaction chromatography

Gel Filtration chromatography (Part 1)

Gel Filtration chromatography (Part 2)

Affinity Chromatography (Part 1)